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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA001311LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA001311LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA001311LA01HU at dilution of 1:100

ADAMTS4 Antibody

CSB-PA001311LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetADAMTS4
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADAMTS4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9507
  • Target name
    ADAMTS4
  • Target description
    ADAM metallopeptidase with thrombospondin type 1 motif 4
  • Target synonyms
    ADAMTS-2, ADAMTS-4, ADMP-1, A disintegrin and metalloproteinase with thrombospondin motifs 4, ADAM-TS 4, ADAM-TS4, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, aggrecanase-1, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75173
  • Protein Name
    A disintegrin and metalloproteinase with thrombospondin motifs 4
  • Scientific Description
    Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the 392-Glu- - -Ala-393 site.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADAMTS4 using anti-ADAMTS4 antibody (A02899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human ADAMTS4 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS4 antigen affinity purified polyclonal antibody (Catalog # A02899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS4 at approximately 58KD. The expected band size for ADAMTS4 is at 53KD.
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