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Mouse Cerebrum lysates probed with ADAMTS4 Polyclonal Antibody, Unconjugated (bs-4191R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Cerebrum lysates probed with ADAMTS4 Polyclonal Antibody, Unconjugated (bs-4191R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Cerebrum lysates probed with ADAMTS4 Polyclonal Antibody, Unconjugated (bs-4191R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

ADAMTS4 Polyclonal Antibody

BS-4191R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetADAMTS4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ADAMTS4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9507
  • Target name
    ADAMTS4
  • Target description
    ADAM metallopeptidase with thrombospondin type 1 motif 4
  • Target synonyms
    A disintegrin and metalloproteinase with thrombospondin motifs 4; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM-TS 4; ADAMTS-2; ADAM-TS4; ADAMTS-4; ADMP-1; aggrecanase-1; epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75173
  • Protein Name
    A disintegrin and metalloproteinase with thrombospondin motifs 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Liu Y et al., 2019, Oxid Med Cell Longev
    Read more
  • LncRNA SNHG1 alleviates IL-1beta-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-kappaB signaling pathways. Lei J et al., 2019 Sep 30, Biosci Rep
    Read more

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Figure 1. Western blot analysis of ADAMTS4 using anti-ADAMTS4 antibody (A02899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human ADAMTS4 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS4 antigen affinity purified polyclonal antibody (Catalog # A02899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS4 at approximately 58KD. The expected band size for ADAMTS4 is at 53KD.
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