ADAR1 antibody [N3C1], Internal detects ADAR1 protein at nucleolus and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ADAR1 protein stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
![Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [N3C1], Internal (GTX101602). Western blot analysis was performed using ADAR1 antibody [N3C1], Internal (GTX101602). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101602/GTX101602_40506_20150724_IP_w_23060100_120.webp "Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [N3C1], Internal (GTX101602). Western blot analysis was performed using ADAR1 antibody [N3C1], Internal (GTX101602). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![ADAR1 antibody [N3C1], Internal detects ADAR1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ADAR1 stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101602/GTX101602_44552_20220325_IHC-P_R_w_23060100_302.webp "ADAR1 antibody [N3C1], Internal detects ADAR1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ADAR1 stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101602/GTX101602_40506_20240802_WB_shRNA_watermark_24080602_392.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101602/GTX101602_45483_20240802_WB_24110700_484.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
ADAR1 antibody [N3C1], Internal detects ADAR1 protein at nucleolus and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ADAR1 protein stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
ADAR1 antibody [N3C1], Internal
GTX101602
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetADAR
Overview
- SupplierGeneTex
- Product NameADAR1 antibody [N3C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.4 mg/ml
- ConjugateUnconjugated
- Gene ID103
- Target nameADAR
- Target descriptionadenosine deaminase RNA specific
- Target synonymsADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136, double-stranded RNA-specific adenosine deaminase, 136 kDa double-stranded RNA-binding protein, adenosine deaminase acting on RNA 1-A, dsRNA adenosine deaminase, dsRNA adeonosine deaminase, interferon-induced protein 4, interferon-inducible protein 4
- HostRabbit
- IsotypeIgG
- Protein IDP55265
- Protein NameDouble-stranded RNA-specific adenosine deaminase
- Scientific DescriptionThis gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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