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ADAR antibody

70R-5881
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    ADAR antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR1/ADAR antigen affinity purified polyclonal antibody (Catalog # A00869-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAR1/ADAR at approximately 110 kDa. The expected band size for ADAR1/ADAR is at 136 kDa.
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Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, LO2 whole cell lysate All lanes: ADAR antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 137, 134, 132, 141, 104 kDa Observed band size: 104 kDa
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