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ADAR1 Antibody (YA1658)

HY-P81913
MedChem Express
TargetADAR
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ADAR1 Antibody (YA1658)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID103
  • Target name
    ADAR
  • Target description
    adenosine deaminase RNA specific
  • Target synonyms
    136 kDa double-stranded RNA-binding protein; ADAR1; adenosine deaminase acting on RNA 1-A; AGS6; double-stranded RNA-specific adenosine deaminase; DRADA; DSH; DSRAD; dsRNA adenosine deaminase; dsRNA adeonosine deaminase; G1P1; IFI4; IFI-4; interferon-induced protein 4; interferon-inducible protein 4; K88DSRBP; P136
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ADAR1 Antibody (YA1658) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1658), targeting ADAR1, with a predicted molecular weight of 136 kDa (observed band size: 150 kDa). ADAR1 Antibody (YA1658) can be used for WB, IHC-P, ICC/IF, FC experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR1/ADAR antigen affinity purified polyclonal antibody (Catalog # A00869-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAR1/ADAR at approximately 110 kDa. The expected band size for ADAR1/ADAR is at 136 kDa.
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