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WB analysis of indicated lysates (35ug per lane) using ADK antibody at a dilution of 1:1,000.
WB analysis of indicated lysates (35ug per lane) using ADK antibody at a dilution of 1:1,000.
WB analysis of indicated lysates (35ug per lane) using ADK antibody at a dilution of 1:1,000.

Adenosine kinase antibody [AT4F8]

GTX53683
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetADK
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Overview

  • Supplier
    GeneTex
  • Product Name
    Adenosine kinase antibody [AT4F8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT4F8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID132
  • Target name
    ADK
  • Target description
    adenosine kinase
  • Target synonyms
    adenosine 5'-phosphotransferase; adenosine kinase; AK; testicular tissue protein Li 14
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP55263
  • Protein Name
    Adenosine kinase
  • Scientific Description
    This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADK using anti-ADK antibody (PB10031). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HepG2 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADK antigen affinity purified polyclonal antibody (Catalog # PB10031) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADK at approximately 45 kDa. The expected band size for ADK is at 45 kDa.
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