Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody [N1C1] (GTX101372) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Adenosine kinase antibody [N1C1] detects Adenosine kinase protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] (GTX101372) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101372/GTX101372_40583_IFA_w_23060100_952.webp "Adenosine kinase antibody [N1C1] detects Adenosine kinase protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] (GTX101372) diluted at 1:500. Blue: Hoechst 33342 staining.")
Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody [N1C1] (GTX101372) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Adenosine kinase antibody [N1C1]
GTX101372
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetADK
Overview
- SupplierGeneTex
- Product NameAdenosine kinase antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID132
- Target nameADK
- Target descriptionadenosine kinase
- Target synonymsAK, adenosine kinase, adenosine 5'-phosphotransferase, testicular tissue protein Li 14
- HostRabbit
- IsotypeIgG
- Protein IDP55263
- Protein NameAdenosine kinase
- Scientific DescriptionThis gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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