Bio-Connect

Adenosine kinase antibody [N1C1]

GTX101372
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetADK
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    Adenosine kinase antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID132
  • Target name
    ADK
  • Target description
    adenosine kinase
  • Target synonyms
    adenosine 5'-phosphotransferase; adenosine kinase; AK; testicular tissue protein Li 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55263
  • Protein Name
    Adenosine kinase
  • Scientific Description
    This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetADK
  • SizePrice
WB analysis of indicated lysates (35ug per lane) using ADK antibody at a dilution of 1:1,000.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
TargetADK
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetADK
  • SizePrice
Product group Antibodies
Assay Genie
ADK AntibodyCAB15023
TargetADK
  • SizePrice
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, mouse intestinum tenue tissue, Primary antibody: CSB-PA152141(ADK Antibody) at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
Product group Antibodies
Cusabio
ADK AntibodyCSB-PA152141
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetADK
  • SizePrice
Product group Antibodies
RayBiotech
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetADK
  • SizePrice
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetADK
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
TargetADK
  • SizePrice
Figure 1. Western blot analysis of ADK using anti-ADK antibody (PB10031). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HepG2 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADK antigen affinity purified polyclonal antibody (Catalog # PB10031) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADK at approximately 45 kDa. The expected band size for ADK is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-ADK Antibody Picoband(r)PB10031-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetADK
  • SizePrice