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Western blot All lanes: Alcohol dehydrogenase 4 antibody at 2ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 43 kDa Observed band size: 41 kDa
Western blot All lanes: Alcohol dehydrogenase 4 antibody at 2ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 43 kDa Observed band size: 41 kDa
Western blot All lanes: Alcohol dehydrogenase 4 antibody at 2ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 43 kDa Observed band size: 41 kDa

ADH4 Antibody

CSB-PA001356ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetADH4
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADH4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID127
  • Target name
    ADH4
  • Target description
    alcohol dehydrogenase 4 (class II), pi polypeptide
  • Target synonyms
    ADH-2; alcohol dehydrogenase class II pi chain; aldehyde reductase; all-trans-retinol dehydrogenase [NAD(+)] ADH4; epididymis secretory protein Li 4; HEL-S-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08319
  • Protein Name
    Alcohol dehydrogenase 4
  • Scientific Description
    This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADH4 using anti-ADH4 antibody (PB10030). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH4 antigen affinity purified polyclonal antibody (Catalog # PB10030) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH4 at approximately 40 kDa. The expected band size for ADH4 is at 40 kDa.
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