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ADH4 Recombinant Antibody

BSM-62543R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetADH4
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Overview

  • Supplier
    Bioss
  • Product Name
    ADH4 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID127
  • Target name
    ADH4
  • Target description
    alcohol dehydrogenase 4 (class II), pi polypeptide
  • Target synonyms
    ADH-2, HEL-S-4, all-trans-retinol dehydrogenase [NAD(+)] ADH4, alcohol dehydrogenase 2, alcohol dehydrogenase class II pi chain, aldehyde reductase, epididymis secretory protein Li 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08319
  • Protein Name
    Alcohol dehydrogenase 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADH4 using anti-ADH4 antibody (PB10030). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. Lane 3: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH4 antigen affinity purified polyclonal antibody (Catalog # PB10030) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH4 at approximately 40 kDa. The expected band size for ADH4 is at 40 kDa.
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