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Adipose Triglyceride Lipase Antibody (YA637, Rabbit)

HY-P80533
MedChem Express
TargetPNPLA2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Adipose Triglyceride Lipase Antibody (YA637, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID57104
  • Target name
    PNPLA2
  • Target description
    patatin like phospholipase domain containing 2
  • Target synonyms
    1110001C14Rik; adipose triglyceride lipase; ATGL; calcium-independent phospholipase A2; desnutrin; FP17548; iPLA2zeta; IPLA2-zeta; mutant patatin-like phospholipase domain containing 2; patatin-like phospholipase domain-containing protein 2; PEDF-R; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; TTS2; TTS2.2; TTS-2.2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Adipose Triglyceride Lipase Antibody (YA637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adipose Triglyceride Lipase.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATGL/PNPLA2 using anti-ATGL/PNPLA2 antibody (M01800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATGL/PNPLA2 antigen affinity purified monoclonal antibody (Catalog # M01800) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATGL/PNPLA2 at approximately 55 kDa. The expected band size for ATGL/PNPLA2 is at 55 kDa.
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Western blot All lanes: PNPLA2 antibody at 10microg/ml Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 20 kDa Observed band size: 56 kDa
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A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ Adipose Triglyceride Lipase Antibody（bs-3831R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & lipid droplets.
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