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Western Blot Positive WB detected in: Mouse brain tissue All lanes: ADORA2B antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: ADORA2B antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: ADORA2B antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa

ADORA2B Antibody

CSB-PA001377LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetADORA2B
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADORA2B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID136
  • Target name
    ADORA2B
  • Target description
    adenosine A2b receptor
  • Target synonyms
    ADORA2, adenosine receptor A2b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29275
  • Protein Name
    Adenosine receptor A2b
  • Scientific Description
    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADORA2B using anti-ADORA2B antibody (A05238-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADORA2B antigen affinity purified polyclonal antibody (Catalog # A05238-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADORA2B at approximately 41 kDa. The expected band size for ADORA2B is at 36 kDa.
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