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Western Blot analysis of HT29 cells using AR-beta1 Polyclonal Antibody
Western Blot analysis of HT29 cells using AR-beta1 Polyclonal Antibody
Western Blot analysis of HT29 cells using AR-beta1 Polyclonal Antibody

ADRB1 Antibody

CSB-PA000937
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetADRB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADRB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID153
  • Target name
    ADRB1
  • Target description
    adrenoceptor beta 1
  • Target synonyms
    ADRB1R; adrenergic, beta-1-, receptor; B1AR; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; BETA1AR; FNSS2; RHR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08588
  • Protein Name
    Beta-1 adrenergic receptor
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Mouse Kidney cells were probed with ADRB1 Polyclonal Antibody, FITC Conjugated (bs-0498R-FITC) 1:200, 40 minutes at 37°C. Primary antibody (red line) is graphed against Isotype Control (bs-0295P-FITC; green line), which was incubated at the same conditions.
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Figure 1. Western blot analysis of ADRB1 using anti-ADRB1 antibody (A00675-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat NRK whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRB1 antigen affinity purified polyclonal antibody (Catalog # A00675-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRB1 at approximately 51 kDa. The expected band size for ADRB1 is at 51 kDa.
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