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Goat anti-ADRB1

EB07133
Everest Biotech
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetADRB1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ADRB1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the plama membrane and cytoplasm of HeLa cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID153
  • Target name
    ADRB1
  • Target description
    adrenoceptor beta 1
  • Target synonyms
    ADRB1R, B1AR, BETA1AR, FNSS2, RHR, beta-1 adrenergic receptor, adrenergic, beta-1-, receptor, beta-1 adrenoceptor, beta-1 adrenoreceptor
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000675.1. Purification: Antigen affinity purified. Names and symbols: ADRB1; adrenergic, beta-1-, receptor; ADRB1R; B1AR; BETA1AR; RHR; beta-1-adrenergic receptor
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Functional autoantibodies in patients with different forms of dementia. Wallukat G et al., 2018, PLoS One
    Read more
  • Difference between beta1-adrenoceptor autoantibodies of human and animal origin-Limitations detecting beta1-adrenoceptor autoantibodies using peptide based ELISA technology. Wenzel K et al., 2018, PLoS One
    Read more
  • Performance and in-house validation of a bioassay for the determination of beta1-autoantibodies found in patients with cardiomyopathy. Wenzel K et al., 2017 Jul, Heliyon
    Read more
  • The first aptamer-apheresis column specifically for clearing blood of beta1-receptor autoantibodies. Wallukat G et al., 2012, Circ J
    Read more
  • Aptamer neutralization of beta1-adrenoceptor autoantibodies isolated from patients with cardiomyopathies. Haberland A et al., 2011 Oct 14, Circ Res
    Read more
  • Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas patients. Wallukat G et al., 2010 Feb 2, J Am Coll Cardiol
    Read more

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Figure 1. Western blot analysis of ADRB1 using anti-ADRB1 antibody (A00675-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat NRK whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRB1 antigen affinity purified polyclonal antibody (Catalog # A00675-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRB1 at approximately 51 kDa. The expected band size for ADRB1 is at 51 kDa.
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