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AF10 Antibody (YA2017, Rabbit)

HY-P82272
MedChem Express
TargetMLLT10
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    AF10 Antibody (YA2017, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8028
  • Target name
    MLLT10
  • Target description
    MLLT10 histone lysine methyltransferase DOT1L cofactor
  • Target synonyms
    AF10, protein AF-10, ALL1-fused gene from chromosome 10 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 10, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10, type I AF10 protein, type III AF10 protein, type IV AF10 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    AF10 Antibody (YA2017) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2017), targeting AF10, with a predicted molecular weight of 113 kDa (observed band size: 140 kDa). AF10 Antibody (YA2017) can be used for WB, IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MLLT10 using anti-MLLT10 antibody (A04471-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLLT10 antigen affinity purified polyclonal antibody (Catalog # A04471-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLLT10 at approximately 130 kDa. The expected band size for MLLT10 is at 113 kDa.
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