Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (GTX637225) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (GTX637225) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637225/GTX637225_44795_20220908_WB_TPM_watermark_22092119_775.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (GTX637225) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![AF9 antibody [HL1644] detects AF9 protein at nucleus and granule by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 stained by AF9 antibody [HL1644] (GTX637225) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637225/GTX637225_T-44746_20220902_ICC_IF_22101718_581.webp "AF9 antibody [HL1644] detects AF9 protein at nucleus and granule by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 stained by AF9 antibody [HL1644] (GTX637225) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (GTX637225) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
AF9 antibody [HL1644]
GTX637225
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMLLT3
Overview
- SupplierGeneTex
- Product NameAF9 antibody [HL1644]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1644
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4300
- Target nameMLLT3
- Target descriptionMLLT3 super elongation complex subunit
- Target synonymsAF9, YEATS3, protein AF-9, ALL1-fused gene from chromosome 9 protein, KMT2A/MLLT3 fusion protein, YEATS domain-containing protein 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3, myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, myeloid/lymphoid or mixed-lineage leukemia; translocated to 3
- HostRabbit
- IsotypeIgG
- Protein IDP42568
- Protein NameProtein AF-9
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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