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Western blot All lanes: AFG3L2 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 89 kDa Observed band size: 89 kDa
Western blot All lanes: AFG3L2 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 89 kDa Observed band size: 89 kDa
Western blot All lanes: AFG3L2 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 89 kDa Observed band size: 89 kDa

AFG3L2 Antibody

CSB-PA04005A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAFG3L2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AFG3L2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10939
  • Target name
    AFG3L2
  • Target description
    AFG3 like matrix AAA peptidase subunit 2
  • Target synonyms
    AFG3 ATPase family gene 3-like 2; AFG3 ATPase family member 3-like 2; AFG3 like AAA ATPase 2; AFG3-like protein 2; ATPase family gene 3, yeast; OPA12; paraplegin-like protein; SCA28; SPAX5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4W6
  • Protein Name
    AFG3-like protein 2
  • Scientific Description
    ATP-dependent protease which is essential for axonal and neuron development. In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU (PubMed:27642048). Required for the maturation of paraplegin (SPG7) after its cleavage by mitochondrial-processing peptidase (MPP), converting it into a proteolytically active mature form (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AFG3L2 using anti-AFG3L2 antibody (PB9915). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFG3L2 antigen affinity purified polyclonal antibody (Catalog # PB9915) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFG3L2 at approximately 89 kDa. The expected band size for AFG3L2 is at 89 kDa.
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