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ProductsBack/AF/LE Purified Anti-Mouse CD48 Antibody

AF/LE Purified Anti-Mouse CD48 Antibody

RD20316F
Reddot Biotech
ApplicationsFlow Cytometry, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetCd48
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    AF/LE Purified Anti-Mouse CD48 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HM48-1
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12506
  • Target name
    Cd48
  • Target description
    CD48 antigen
  • Target synonyms
    BCM1, BLAST, BLAST-1, BLAST1, Bcm-1, MEM-102, SLAMF2, Sgp-60, CD48 antigen, BCM1 surface antigen, HM48-1, MRC OX-45 surface antigen, SLAM family member 2, signaling lymphocytic activation molecule 2
  • Host
    Hamster
  • Isotype
    IgG
  • Scientific Description
    CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd48 using anti-Cd48 antibody (A03281-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd48 antigen affinity purified polyclonal antibody (Catalog # A03281-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd48 at approximately 50 kDa. The expected band size for Cd48 is at 28,45-50 kDa.
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