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ProductsBack/AFP (Alpha Fetoprotein)(C3), CF405S conjugate, 0.1mg/mL [26628-22-8]
AFP (Alpha Fetoprotein)(C3), CF405S conjugate, 0.1mg/mL [26628-22-8]

AFP (Alpha Fetoprotein)(C3), CF405S conjugate, 0.1mg/mL [26628-22-8]

BNC040354
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAFP
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Overview

  • Supplier
    Biotium
  • Product Name
    AFP (Alpha Fetoprotein)(C3), CF405S conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C3
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID174
  • Target name
    AFP
  • Target description
    alpha fetoprotein
  • Target synonyms
    AFPD; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-fetoprotein; FETA; HPAFP
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP02771
  • Protein Name
    Alpha-fetoprotein
  • Scientific Description
    Alpha-fetoprotein (AFP) is an oncofetal glycoprotein with a single chain of 70 kDa. This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of alpha 1 Fetoprotein/AFP using anti-alpha 1 Fetoprotein/AFP antibody (A00522-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 1 Fetoprotein/AFP antigen affinity purified polyclonal antibody (Catalog # A00522-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 1 Fetoprotein/AFP at approximately 69 kDa. The expected band size for alpha 1 Fetoprotein/AFP is at 69 kDa.
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