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Western blot All lanes: Afp antibody at 10microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Rat liver tissue Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa
Western blot All lanes: Afp antibody at 10microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Rat liver tissue Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa
Western blot All lanes: Afp antibody at 10microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Rat liver tissue Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa

Afp Antibody

CSB-PA001421LA01MO
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAfp
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Overview

  • Supplier
    Cusabio
  • Product Name
    Afp Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11576
  • Target name
    Afp
  • Target description
    alpha fetoprotein
  • Target synonyms
    alpha-fetoprotein, alpha-1-fetoprotein, alpha-fetoglobulin, alpha-foetoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02772
  • Protein Name
    Alpha-fetoprotein
  • Scientific Description
    Binds estrogens, fatty acids and metals.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Alpha 1 Fetoprotein using anti-Alpha 1 Fetoprotein antibody (A00522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Fetoprotein antigen affinity purified polyclonal antibody (Catalog # A00522) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Fetoprotein at approximately 69KD. The expected band size for Alpha 1 Fetoprotein is at 69KD.
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Lane 1: Mouse Embryo tissue lysates; Lane 2: Rat Embryo tissue lysates probed with alpha 1 Fetoprotein Polyclonal Antibody, Unconjugated (bs-8616R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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