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ProductsBack/alpha 1 Fetoprotein Polyclonal Antibody
Lane 1: Mouse Embryo tissue lysates; Lane 2: Rat Embryo tissue lysates probed with alpha 1 Fetoprotein Polyclonal Antibody, Unconjugated (bs-8616R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Embryo tissue lysates; Lane 2: Rat Embryo tissue lysates probed with alpha 1 Fetoprotein Polyclonal Antibody, Unconjugated (bs-8616R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Embryo tissue lysates; Lane 2: Rat Embryo tissue lysates probed with alpha 1 Fetoprotein Polyclonal Antibody, Unconjugated (bs-8616R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

alpha 1 Fetoprotein Polyclonal Antibody

BS-8616R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetAfp
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Overview

  • Supplier
    Bioss
  • Product Name
    alpha 1 Fetoprotein Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID11576
  • Target name
    Afp
  • Target description
    alpha fetoprotein
  • Target synonyms
    alpha-fetoprotein, alpha-1-fetoprotein, alpha-fetoglobulin, alpha-foetoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Alpha 1 Fetoprotein using anti-Alpha 1 Fetoprotein antibody (A00522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Fetoprotein antigen affinity purified polyclonal antibody (Catalog # A00522) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Fetoprotein at approximately 69KD. The expected band size for Alpha 1 Fetoprotein is at 69KD.
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