More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-RAGE [2A11]

Anti-RAGE [2A11]

Ab01230-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Other Application
Product group Antibodies
TargetAGER
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-RAGE [2A11]
  • Delivery Days Customer
    7
  • Application Supplier Note
    ELISA confirmed the specificity of 2A11 for the V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to RAGE is glycosylation independent. In SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-RAGE cells.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Other Application
  • Applications Supplier
    Western blot; IHC; IF; ELISA; FC; SPR
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A11
  • Gene ID177
  • Target name
    AGER
  • Target description
    advanced glycosylation end-product specific receptor
  • Target synonyms
    advanced glycosylation end product-specific receptor; RAGE; receptor for advanced glycation end-products variant 20; SCARJ1; sRAGE
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15109
  • Protein Name
    Advanced glycosylation end product-specific receptor
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Human RAGE protein.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE/AGER antigen affinity purified polyclonal antibody (Catalog # A03438-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE/AGER at approximately 45-58 kDa. The expected band size for RAGE/AGER is at 43 kDa.
Product group Antibodies
Boster Bio
Anti-RAGE/AGER Antibody Picoband(r)A03438-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetAGER
  • SizePrice
Immunohistochemistry analysis in human lung and prostate tissues using AMAb91634 antibody. Corresponding AGER RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-AGER AntibodyAMAB91634
ApplicationsImmunoHistoChemistry
ReactivityHuman, Rat
TargetAGER
  • SizePrice
WB analysis of mouse lung tissue lysate (35ug/lane) using GTX16592 RAGE antibody.
Product group Antibodies
GeneTex
RAGE antibodyGTX16592
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetAGER
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-AGERY058663
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Assay Genie
AGER AntibodyCAB13264
TargetAGER
  • SizePrice
Western Blot analysis of 3T3 cells using RAGE Polyclonal Antibody
Product group Antibodies
Cusabio
AGER AntibodyCSB-PA007810
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetAGER
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
AGER / RAGE AntibodyLS-C400434
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetAGER
  • SizePrice
Product group Antibodies
Biomatik
Receptor For Advanced Glycation Endproducts (RAGE) Polyclonal AntibodyCAU27462
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetAGER
  • SizePrice
Mouse lung lysates probed with RAGE Polyclonal Antibody, Unconjugated (bs-4999R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss Antibodies
RAGE Polyclonal AntibodyBS-4999R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetAGER
  • SizePrice
Product group Antibodies
Biosynth
RAGE antibody20R-RG001
  • SizePrice