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AGO2 Antibody

CSB-PA871064XA01DOA
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityPlant
TargetAGO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AGO2 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID840016
  • Target name
    AGO2
  • Target description
    Argonaute family protein
  • Target synonyms
    AT1G31280, AtAGO2, T19E23.7, T19E23_7, argonaute 2, Argonaute family protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9SHF3
  • Protein Name
    Protein argonaute 2
  • Reactivity
    Plant
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Ago2/eIF2C2 using anti-Ago2/eIF2C2 antibody (PB9978). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ago2/eIF2C2 antigen affinity purified polyclonal antibody (Catalog # PB9978) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ago2/eIF2C2 at approximately 97 kDa. The expected band size for Ago2/eIF2C2 is at 97 kDa.
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