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AGO3 / EIF2C3 Antibody

LS-C752079
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAGO3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AGO3 / EIF2C3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID192669
  • Target name
    AGO3
  • Target description
    argonaute RISC catalytic component 3
  • Target synonyms
    EIF2C3, protein argonaute-3, argonaute 3, RISC catalytic component, eukaryotic translation initiation factor 2C, 3, hAgo3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AGO3 using anti-AGO3 antibody (A04191-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO3 antigen affinity purified polyclonal antibody (Catalog # A04191-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGO3 at approximately 97KD. The expected band size for AGO3 is at 97KD.
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