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ProductsBack/Argonaute 3/eIF2C3 Recombinant Antibody, Biotin Conjugated

Argonaute 3/eIF2C3 Recombinant Antibody, Biotin Conjugated

BSM-61315R-BIOTIN
Bioss Antibodies
ApplicationsWestern Blot
Product group Antibodies
TargetAGO3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Argonaute 3/eIF2C3 Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID192669
  • Target name
    AGO3
  • Target description
    argonaute RISC catalytic component 3
  • Target synonyms
    argonaute 3, RISC catalytic component; EIF2C3; eukaryotic translation initiation factor 2C, 3; hAgo3; protein argonaute-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H9G7
  • Protein Name
    Protein argonaute-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AGO3 using anti-AGO3 antibody (A04191-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO3 antigen affinity purified polyclonal antibody (Catalog # A04191-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGO3 at approximately 97KD. The expected band size for AGO3 is at 97KD.
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