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Ahcyl1 Polyclonal Antibody

CAC10683
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAHCYL1
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Overview

  • Supplier
    Biomatik
  • Product Name
    AHCYL1 Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10768
  • Target name
    AHCYL1
  • Target description
    adenosylhomocysteinase like 1
  • Target synonyms
    DCAL, IRBIT, PPP1R78, PRO0233, XPVKONA, S-adenosylhomocysteine hydrolase-like protein 1, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), S-adenosyl homocysteine hydrolase homolog, S-adenosyl-L-homocysteine hydrolase 2, adenosylhomocysteinase 2, adoHcyase 2, dendritic cell expressed AHCY-like protein, epididymis secretory sperm binding protein, inositol 1,4,5-trisphosphate receptor-binding protein, protein phosphatase 1, regulatory subunit 78, putative adenosylhomocysteinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43865
  • Protein Name
    S-adenosylhomocysteine hydrolase-like protein 1
  • Scientific Description
    The Ahcyl1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRBIT/AHCYL1 using anti-IRBIT/AHCYL1 antibody (A08908-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRBIT/AHCYL1 antigen affinity purified polyclonal antibody (Catalog # A08908-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRBIT/AHCYL1 at approximately 61 kDa. The expected band size for IRBIT/AHCYL1 is at 61 kDa.
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