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Immunohistochemical staining of human duodenum shows moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate membranous and cytoplasmic positivity in glandular cells.

Anti-AHCYL1-25ul

HPA042589
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AHCYL1-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ICC, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43865
  • Protein Name
    S-adenosylhomocysteine hydrolase-like protein 1
  • Scientific Description
    Rabbit Polyclonal Anti-AHCYL1 Antibody against Human adenosylhomocysteinase-like 1. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Western blot All lanes: AHCYL1 antibody at 12ug/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59, 54 kDa Observed band size: 59 kDa
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Figure 1. Western blot analysis of IRBIT/AHCYL1 using anti-IRBIT/AHCYL1 antibody (A08908-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRBIT/AHCYL1 antigen affinity purified polyclonal antibody (Catalog # A08908-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRBIT/AHCYL1 at approximately 61 kDa. The expected band size for IRBIT/AHCYL1 is at 61 kDa.
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