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AHR antibody

GTX129012
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAHR
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Overview

  • Supplier
    GeneTex
  • Product Name
    AHR antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID196
  • Target name
    AHR
  • Target description
    aryl hydrocarbon receptor
  • Target synonyms
    FVH3, RP85, bHLHe76, aryl hydrocarbon receptor, AH-receptor, ah receptor, aromatic hydrocarbon receptor, class E basic helix-loop-helix protein 76
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35869
  • Protein Name
    Aryl hydrocarbon receptor
  • Scientific Description
    This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wang TH, Huang KY, Chen CC, et al. PM2.5 promotes lung cancer progression through activation of the AhR-TMPRSS2-IL18 pathway. EMBO Mol Med. 2023,15(6):e17014. doi: 10.15252/emmm.202217014
    Read this paper
  • Chen CS, Gao GL, Ho DR, et al. Cyproterone acetate acts as a disruptor of the aryl hydrocarbon receptor. Sci Rep. 2021,11(1):5457. doi: 10.1038/s41598-021-84769-7
    Read this paper
  • Wang HC, Liu KY, Wang LT, et al. Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells. Hum Cell. 2021,34(3):785-799. doi: 10.1007/s13577-021-00491-6
    Read this paper

Datasheet

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Figure 1. Western blot analysis of AHR using anti-AHR antibody (A00225-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # A00225-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 100 kDa. The expected band size for AHR is at 96 kDa.
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