![AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500. Blue: Hoechst 33343 staining. AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500. Blue: Hoechst 33343 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102399/GTX102399_39925_IFA_w_23060100_355.webp)
AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500. Blue: Hoechst 33343 staining.
AIF antibody [N1N2], N-term
GTX102399
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetAIFM1
Overview
- SupplierGeneTex
- Product NameAIF antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.72 mg/ml
- ConjugateUnconjugated
- Gene ID9131
- Target nameAIFM1
- Target descriptionapoptosis inducing factor mitochondria associated 1
- Target synonymsAIF, AUNX1, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, SEMDHL, apoptosis-inducing factor 1, mitochondrial, apoptosis-inducing factor, mitochondrion-associated, 1, auditory neuropathy, X-linked recessive 1, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor, testicular secretory protein Li 4
- HostRabbit
- IsotypeIgG
- Protein IDO95831
- Protein NameApoptosis-inducing factor 1, mitochondrial
- Scientific DescriptionThis gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Adriaenssens E, Asselbergh B, Rivera-Mejías P, et al. Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space. Nat Cell Biol. 2023,25(3):467-480. doi: 10.1038/s41556-022-01074-9Read this paper
![AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5 % SDS-PAGE AIF antibody [N1N2], N-term (GTX102399) dilution: 1:1000 AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5 % SDS-PAGE AIF antibody [N1N2], N-term (GTX102399) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX102399/GTX102399_39925_WB_w_23060100_227.webp)

![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term (GTX102399) diluted at 1:4000. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1N2], N-term (GTX102399) diluted at 1:4000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102399/GTX102399_39925_20160804_WB_shRNA_watermark_w_23060100_700.webp)

![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [GT1143] (GTX00833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00833/GTX00833_4000000015_20200410_WB_w_23053121_106.webp)


![WB analysis of NIH3T3 (1), Jurkat (2), HeLa (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate using GTX60476 AIF antibody [4E7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60476/GTX60476_20170912_WB_w_23061123_588.webp)


