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K562 lysates probed with AIF Polyclonal Antibody, unconjugated (bs-0037R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
K562 lysates probed with AIF Polyclonal Antibody, unconjugated (bs-0037R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
K562 lysates probed with AIF Polyclonal Antibody, unconjugated (bs-0037R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

AIF Polyclonal Antibody

BS-0037R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAIFM1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    AIF Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9131
  • Target name
    AIFM1
  • Target description
    apoptosis inducing factor mitochondria associated 1
  • Target synonyms
    AIF; apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; auditory neuropathy, X-linked recessive 1; AUNX1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; programmed cell death 8 (apoptosis-inducing factor); SEMDHL; striatal apoptosis-inducing factor; testicular secretory protein Li 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95831
  • Protein Name
    Apoptosis-inducing factor 1, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ginsenoside Rg3 Prevents Cognitive Impairment by Improving Mitochondrial Dysfunction in the Rat Model of Alzheimers Disease. Zhang Y et al., 2019 Sep 11, J Agric Food Chem
    Read more
  • Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Zhang H et al., 2019, Am J Transl Res
    Read more
  • The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats. Zhu B et al., 2019 Jun 15, Int J Biol Macromol
    Read more
  • Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimers Disease Mouse Model. Li Z et al., 2017 Jul 27, Int J Mol Sci
    Read more
  • Effect of cilostazol pretreatment on the PARP/AIF-mediated apoptotic pathway in rat cerebral ischemia-reperfusion models. Ba XH et al., 2014 May, Exp Ther Med
    Read more

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Figure 3. IHC analysis of AIF using anti-AIF antibody (PB9366). AIF was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-AIF Antibody (PB9366) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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