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Western blot All lanes: AK1 antibody at 0.51 ug/ml Lane 1: Mouse skeletal muscle tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Western blot All lanes: AK1 antibody at 0.51 ug/ml Lane 1: Mouse skeletal muscle tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Western blot All lanes: AK1 antibody at 0.51 ug/ml Lane 1: Mouse skeletal muscle tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa

AK1 Antibody

CSB-PA001508DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    AK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID203
  • Target name
    AK1
  • Target description
    adenylate kinase 1
  • Target synonyms
    adenylate kinase isoenzyme 1; adenylate monophosphate kinase; ATP:AMP phosphotransferase; ATP-AMP transphosphorylase 1; epididymis secretory sperm binding protein; HTL-S-58j; myokinase; testis secretory sperm binding protein Li 58j
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00568
  • Protein Name
    Adenylate kinase isoenzyme 1
  • Scientific Description
    Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AK1 using anti-AK1 antibody (PB10033). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AK1 antigen affinity purified polyclonal antibody (Catalog # PB10033) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AK1 at approximately 22 kDa. The expected band size for AK1 is at 22 kDa.
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