
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AKAP12 Polyclonal Antibody, Unconjugated (bs-1381R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
AKAP12 Polyclonal Antibody
BS-1381R

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAKAP12
Overview
- SupplierBioss Antibodies
- Product NameAKAP12/Gravin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID9590
- Target nameAKAP12
- Target descriptionA-kinase anchoring protein 12
- Target synonymsA kinase (PRKA) anchor protein 12; AKAP 250; AKAP250; A-kinase anchor protein 12; A-kinase anchor protein, 250kDa; kinase scaffold protein gravin; myasthenia gravis autoantigen gravin; Src-Suppressed C Kinase Substrate; SSeCKS
- HostRabbit
- IsotypeIgG
- Storage Instruction-20°C
- UNSPSC12352203