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AKAP14 Antibody (YA3078, Rabbit)

HY-P83333
MedChem Express
TargetAKAP14
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    AKAP14 Antibody (YA3078, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID158798
  • Target name
    AKAP14
  • Target description
    A-kinase anchoring protein 14
  • Target synonyms
    AKAP28, PRKA14, A-kinase anchor protein 14, A kinase (PRKA) anchor protein 14, A-kinase anchor protein 28 kDa, A-kinase anchoring protein 28, AKAP 28, AKAP-14, protein kinase A-anchoring protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    AKAP14 Antibody (YA3078) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3078), targeting AKAP14, with a predicted molecular weight of 23 kDa (observed band size: 23 kDa). AKAP14 Antibody (YA3078) can be used for WB, ICC/IF, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AKAP14 using anti-AKAP14 antibody (M15886). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP14 antigen affinity purified monoclonal antibody (Catalog # M15886) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP14 at approximately 21,23 kDa. The expected band size for AKAP14 is at 21 kDa.
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