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AKAP2 Antibody

CSB-PA010254
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAKAP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AKAP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11217
  • Target name
    AKAP2
  • Target description
    A-kinase anchoring protein 2
  • Target synonyms
    AKAP-2, AKAPKL, MISP2, PRKA2, A kinase (PRKA) anchor protein 2, protein kinase A anchoring protein 2, protein kinase A2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2D5
  • Protein Name
    PALM2-AKAP2 fusion protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AKAP2 using anti-AKAP2 antibody (A09279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP2 antigen affinity purified polyclonal antibody (Catalog # A09279) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP2 at approximately 150-170 kDa. The expected band size for AKAP2 is at 95 kDa.
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