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ProductsBack/AKR1B10 Antibody
Western blot All lanes: AKR1B10 antibody at 4microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western blot All lanes: AKR1B10 antibody at 4microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western blot All lanes: AKR1B10 antibody at 4microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa

AKR1B10 Antibody

CSB-PA001540ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAKR1B10
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Overview

  • Supplier
    Cusabio
  • Product Name
    AKR1B10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57016
  • Target name
    AKR1B10
  • Target description
    aldo-keto reductase family 1 member B10
  • Target synonyms
    AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI, aldo-keto reductase family 1 member B10, ARP, SI reductase, aldo-keto reductase family 1, member B10 (aldose reductase), aldo-keto reductase family 1, member B11 (aldose reductase-like), aldose reductase-like 1, aldose reductase-like peptide, aldose reductase-related protein, hARP, small intestine reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60218
  • Protein Name
    Aldo-keto reductase family 1 member B10
  • Scientific Description
    Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunohistochemical staining of human small intestine shows strong cytoplasmic, nuclear and membranous positivity in glandular cells.
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Figure 1. Western blot analysis of AKR1B10 using anti-AKR1B10 antibody (A02976). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1B10 antigen affinity purified polyclonal antibody (Catalog # A02976) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1B10 at approximately 36 kDa. The expected band size for AKR1B10 is at 36 kDa.
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