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Immunofluorescence staining of HepG2 cells with CSB-PA001542LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cells with CSB-PA001542LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cells with CSB-PA001542LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

AKR1C1 Antibody

CSB-PA001542LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetAKR1C1
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Overview

  • Supplier
    Cusabio
  • Product Name
    AKR1C1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1645
  • Target name
    AKR1C1
  • Target description
    aldo-keto reductase family 1 member C1
  • Target synonyms
    20 alpha-hydroxysteroid dehydrogenase; 20-ALPHA-HSD; 2-ALPHA-HSD; aldo-keto reductase C; aldo-keto reductase family 1 member C1; C9; chlordecone reductase homolog HAKRC; DD1; DD1/DD2; DDH; DDH1; dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase; H-37; HAKRC; HBAB; hepatic dihydrodiol dehydrogenase; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; MBAB; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04828
  • Protein Name
    Aldo-keto reductase family 1 member C1
  • Scientific Description
    Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKR1C1/C2 using anti-AKR1C1/C2 antibody (PB10036). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HCCP tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mosue liver tissue lysates, Lane 7: mosue kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C1/C2 antigen affinity purified polyclonal antibody (Catalog # PB10036) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1C1/C2 at approximately 37 kDa. The expected band size for AKR1C1/C2 is at 37 kDa.
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