
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3(GTX104627) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
AKR1C3 antibody [C2C3], C-term
GTX104627
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetAKR1C3
Overview
- SupplierGeneTex
- Product NameAKR1C3 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8644
- Target nameAKR1C3
- Target descriptionaldo-keto reductase family 1 member C3
- Target synonymsDD3, DDX, HA1753, HAKRB, HAKRe, HSD17B5, PGFS, hluPGFS, aldo-keto reductase family 1 member C3, 3-alpha hydroxysteroid dehydrogenase, type II, 3-alpha-HSD type II, brain, chlordecone reductase homolog HAKRb, dihydrodiol dehydrogenase 3, dihydrodiol dehydrogenase X, indanol dehydrogenase, prostaglandin F synthase, testosterone 17-beta-dehydrogenase 5, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, type IIb 3-alpha hydroxysteroid dehydrogenase
- HostRabbit
- IsotypeIgG
- Protein IDP42330
- Protein NameAldo-keto reductase family 1 member C3
- Scientific DescriptionThis gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 μg of AKR1C3 antibody [C2C3], C-term (GTX104627). Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term (GTX104627). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 μg of AKR1C3 antibody [C2C3], C-term (GTX104627). Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term (GTX104627). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104627/GTX104627_39624_20150529_IP_w_23060120_206.webp)
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104627/GTX104627_39624_20181221_WB_shRNA_watermark_w_23060120_516.webp)


![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody [C2C3], C-term (GTX104627) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104627/GTX104627_39624_20240322_WB_R_liver_24032600_550.webp)






