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ProductsBack/AKR1C3 Recombinant Antibody, AbBy Fluor-405 Conjugated

AKR1C3 Recombinant Antibody, AbBy Fluor-405 Conjugated

BSM-62035R-BF405
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetAKR1C3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    AKR1C3 Recombinant Antibody, AbBy Fluor-405 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID8644
  • Target name
    AKR1C3
  • Target description
    aldo-keto reductase family 1 member C3
  • Target synonyms
    3-alpha hydroxysteroid dehydrogenase, type II; 3-alpha-HSD type II, brain; aldo-keto reductase family 1 member C3; chlordecone reductase homolog HAKRb; DD3; DDX; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; HA1753; HAKRB; HAKRe; hluPGFS; HSD17B5; indanol dehydrogenase; PGFS; prostaglandin F synthase; testosterone 17-beta-dehydrogenase 5; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type IIb 3-alpha hydroxysteroid dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42330
  • Protein Name
    Aldo-keto reductase family 1 member C3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: AKR1C3 antibody at 8microg/ml Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37, 24 kDa Observed band size: 37 kDa
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Figure 1. Western blot analysis of AKR1C3 using anti-AKR1C3 antibody (A01820-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C3 antigen affinity purified polyclonal antibody (Catalog # A01820-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1C3 at approximately 37 kDa. The expected band size for AKR1C3 is at 37 kDa.
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