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IHC-P analysis of human lung carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.
IHC-P analysis of human lung carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.
IHC-P analysis of human lung carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.

AKT (phospho Ser473) antibody

GTX50128
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAKT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AKT (phospho Ser473) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimers Disease Pathology, Constitute a Potential Object for Early Diagnosis.
    Read more
  • The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma. Chen ZH et al., 2021 Sep 7, Genome Med
    Read more
  • Sclareol ameliorated ERCC1-mediated cisplatin resistance in A549 human lung adenocarcinoma cells and a murine xenograft tumor model by suppressing AKT-GSK3beta-AP1/Snail and JNK-AP1 pathways. Pan CH et al., 2020 Dec 1, Chem Biol Interact
    Read more
  • Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta. Pan CH et al., 2019 Oct 3, Aging (Albany NY)
    Read more
  • PSMD4 is a novel therapeutic target in chemoresistant colorectal cancer activated by cytoplasmic localization of Nrf2. Cheng YM et al., 2018 May 29, Oncotarget
    Read more
  • Orchiectomy and letrozole differentially regulate synaptic plasticity and spatial memory in a manner that is mediated by SRC-1 in the hippocampus of male mice. Zhao J et al., 2018 Apr, J Steroid Biochem Mol Biol
    Read more
  • Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4. Lin PL et al., 2016 Jun, Free Radic Biol Med
    Read more

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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