ProductsBack/AKT (phospho Thr308) antibody

Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4oC. Secondary antibody: DyLight? 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.

AKT (phospho Thr308) antibody

GTX28933

ApplicationsDot Blot, Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityHuman, Mouse, Rat

TargetAKT1

Overview

Supplier
GeneTex
Product Name
AKT (phospho Thr308) antibody
Delivery Days Customer
9
Application Supplier Note
WB: 1:1000. IHC-P: 1:200. ELISA: 1:15000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
Dot Blot, Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Polyclonal
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID207
Target name
AKT1
Target description
AKT serine/threonine kinase 1
Target synonyms
AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
Host
Rabbit
Isotype
IgG
Protein IDP31749
Protein Name
RAC-alpha serine/threonine-protein kinase
Scientific Description
Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Reactivity
Human, Mouse, Rat
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Li H, Xue X, Li L, et al. Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway. Neurotox Res. 2020,37(4):996-1008. doi: 10.1007/s12640-020-00165-5
Read this paper
Liu J, Cao L, Zhao N, et al. miR‑338‑3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β‑catenin signaling pathways. Mol Med Rep. 2019,20(1):33-40. doi: 10.3892/mmr.2019.10215
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Piva MBR, Jakubzig B, Bendas G. Integrin Activation Contributes to Lower Cisplatin Sensitivity in MV3 Melanoma Cells by Inducing the Wnt Signalling Pathway. Cancers (Basel). 2017,9(9). doi: 10.3390/cancers9090125
Read this paper
Chen CY, Lin YS, Chen CL, et al. Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma. Oncotarget. 2015,6(29):26946-59. doi: 10.18632/oncotarget.4521
Read this paper
Matsuda N, Takano Y, Kageyama S, et al. Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. Cardiovasc Res. 2007,76(1):132-40.
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AKT (phospho Thr308) antibody

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