Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4oC. Secondary antibody: DyLight? 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.
Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4oC. Secondary antibody: DyLight? 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.
AKT (phospho Thr308) antibody
GTX28933
ApplicationsDot Blot, Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAKT1
Overview
- SupplierGeneTex
- Product NameAKT (phospho Thr308) antibody
- Delivery Days Customer9
- Antibody SpecificityThis antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT.
- Application Supplier NoteWB: 1:1000. IHC-P: 1:200. ELISA: 1:15000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID207
- Target nameAKT1
- Target descriptionAKT serine/threonine kinase 1
- Target synonymsAKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP31749
- Protein NameRAC-alpha serine/threonine-protein kinase
- Scientific DescriptionAkt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway. Li H et al., 2020 Apr, Neurotox ResRead more
- miR-338-3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and beta-catenin signaling pathways. Liu J et al., 2019 Jul, Mol Med RepRead more
- Integrin Activation Contributes to Lower Cisplatin Sensitivity in MV3 Melanoma Cells by Inducing the Wnt Signalling Pathway. Piva MBR et al., 2017 Sep 16, Cancers (Basel)Read more
- Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma. Chen CY et al., 2015 Sep 29, OncotargetRead more
- Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. Matsuda N et al., 2007 Oct 1, Cardiovasc ResRead more