Figure 1. Detection of endogenous PKB Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab PKB-5C10 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). lane 1: SW480; lane 2: SW620; lane 3: HT29; lane 4: MCF-7; lane 5: MDA-MB 231; lane 6: T47D
Figure 1. Detection of endogenous PKB Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab PKB-5C10 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). lane 1: SW480; lane 2: SW620; lane 3: HT29; lane 4: MCF-7; lane 5: MDA-MB 231; lane 6: T47D
AKT1 (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5C10]
AM00110PU-N
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetAKT1
Overview
- SupplierOriGene
- Product NameAKT1 (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5C10]
- Delivery Days Customer14
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5C10
- Gene ID207
- Target nameAKT1
- Target descriptionAKT serine/threonine kinase 1
- Target synonymsAKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
- HostMouse
- IsotypeIgG1
- Protein IDP31749
- Protein NameRAC-alpha serine/threonine-protein kinase
- Scientific DescriptionAKT1 (C-term) (incl. pos. control) mouse monoclonal antibody, clone 5C10, Purified
- ReactivityCanine, Human, Mouse, Rat
- Storage Instruction-20°C,-80°C,2°C to 8°C
- UNSPSC12352203