Untreated (-) and treated (+) MCF-7 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser124) antibody [GT1268] (GTX03180) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (-) and treated (+) MCF-7 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser124) antibody [GT1268] (GTX03180) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
AKT1 (phospho Ser124) antibody [GT1268]
GTX03180
Overview
- SupplierGeneTex
- Product NameAKT1 (phospho Ser124) antibody [GT1268] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityBased on internal testing result, this antibody does not cross with AKT2 and AKT3 proteins.
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1268
- Concentration1.43 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID207
- Target nameAKT1
- Target descriptionAKT serine/threonine kinase 1
- Target synonymsAKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDP31749
- Protein NameRAC-alpha serine/threonine-protein kinase
- Scientific DescriptionThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203