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Confocal microscopy of PFA-fixed, and LPS-treated for 4h, HeLa (0.1% TX100 permeabilised) stained with AKT1-pS124 Rabbit Polyclonal Antibody AE00296 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the AKT1-pS124 rabbit antibody, AF488 (green) for beta-tubulin mouse antibody, and DAPI (blue) for nuclear staining.
Confocal microscopy of PFA-fixed, and LPS-treated for 4h, HeLa (0.1% TX100 permeabilised) stained with AKT1-pS124 Rabbit Polyclonal Antibody AE00296 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the AKT1-pS124 rabbit antibody, AF488 (green) for beta-tubulin mouse antibody, and DAPI (blue) for nuclear staining.
Confocal microscopy of PFA-fixed, and LPS-treated for 4h, HeLa (0.1% TX100 permeabilised) stained with AKT1-pS124 Rabbit Polyclonal Antibody AE00296 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the AKT1-pS124 rabbit antibody, AF488 (green) for beta-tubulin mouse antibody, and DAPI (blue) for nuclear staining.

AKT1-pS124 Polyclonal Antibody AE00296

AE00296
Aeonian Biotech
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetAKT1
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    AKT1-pS124 Polyclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ICC, IHC, WB
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Scientific Description
    AKT1-pS124 Polyclonal Antibody AE00296
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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