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ProductsBack/AKT1 pSer473 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11E6]
Phosphospecificity: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) SKOV3 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab 11E6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp.time: 30 sec).
Phosphospecificity: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) SKOV3 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab 11E6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp.time: 30 sec).
Phosphospecificity: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) SKOV3 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab 11E6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp.time: 30 sec).

AKT1 pSer473 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11E6]

AM00106PU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetAKT1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    AKT1 pSer473 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11E6]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11E6
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Scientific Description
    AKT1 pSer473 (incl. pos. control) mouse monoclonal antibody, clone 11E6, Purified
  • Reactivity
    Canine, Human, Mouse, Rat
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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