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ProductsBack/AKT1 Polyclonal Antibody
Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/2/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/2/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/2/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

AKT1 Polyclonal Antibody

BS-0115R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetAKT1
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Overview

  • Supplier
    Bioss
  • Product Name
    AKT1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Liu B, Zhang J, Hu S, et al. MicroRNA-379 mediates pigmentation, migration and proliferation of melanocytes by targeting the insulin-like growth factor 1 receptor. Exp Dermatol. 2020,29(5):467-476. doi: 10.1111/exd.14095
    Read this paper
  • Kim K, Park S, Kim H, et al. Enterococcus faecium L-15 Extract Enhances the Self-Renewal and Proliferation of Mouse Skin-Derived Precursor Cells. Probiotics Antimicrob Proteins. 2020,12(4):1492-1501. doi: 10.1007/s12602-020-09635-w
    Read this paper
  • Zhang Y, Sun L, Sun Y, et al. Overexpressed CES2 has prognostic value in CRC and knockdown CES2 reverses L-OHP-resistance in CRC cells by inhibition of the PI3K signaling pathway. Exp Cell Res. 2020,389(1):111856. doi: 10.1016/j.yexcr.2020.111856
    Read this paper
  • Wang J , Xu H , Cheng X , et al. Calcium relieves fluoride-induced bone damage through the PI3K/AKT pathway. Food Funct. 2020,11(1):1155-1164. doi: 10.1039/c9fo02491c
    Read this paper
  • Wang Y, Zhang C, Xu C, et al. H(2)S mediates apoptosis in response to inflammation through PI3K/Akt/NFκB signaling pathway. Biotechnol Lett. 2020,42(3):375-387. doi: 10.1007/s10529-019-02782-3
    Read this paper
  • Kamohara A, Hirata H, Xu X, et al. IgG immune complexes with Staphylococcus aureus protein A enhance osteoclast differentiation and bone resorption by stimulating Fc receptors and TLR2. Int Immunol. 2020,32(2):89-104. doi: 10.1093/intimm/dxz063
    Read this paper
  • Liu F, Wang L, Yi S, et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019,120:109533. doi: 10.1016/j.biopha.2019.109533
    Read this paper
  • Wang Z, Kuang G, Yu Z, et al. Light-activatable dual prodrug polymer nanoparticle for precise synergistic chemotherapy guided by drug-mediated computed tomography imaging. Acta Biomater. 2019,94:459-468. doi: 10.1016/j.actbio.2019.05.047
    Read this paper
  • Zhang H, Su Y, Wang J, et al. Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signaling pathway. Life Sci. 2019,229:210-218. doi: 10.1016/j.lfs.2019.05.033
    Read this paper
  • Tang Q, Ke H, Wu C, et al. Aqueous extract from You-Gui-Yin ameliorates cognitive impairment of chronic renal failure mice through targeting hippocampal CaMKIIα/CREB/BDNF and EPO/EPOR pathways. J Ethnopharmacol. 2019,239:111925. doi: 10.1016/j.jep.2019.111925
    Read this paper

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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