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Western Blot analysis of 293T and Jurkat cell using ALAS2 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of 293T and Jurkat cell using ALAS2 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of 293T and Jurkat cell using ALAS2 Polyclonal Antibody at dilution of 1:1000

ALAS2 Polyclonal Antibody

E-AB-10763
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ALAS2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.2mg/ml
  • Scientific Description
    The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.
  • UNSPSC
    12352203

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Western blot All lanes: 5-aminolevulinate synthase, erythroid-specific, mitochondrial antibody at 7ug/ml Lane 1: 293T whole cell lysate Lane 2: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 65, 61, 50, 64 kDa Observed band size: 65 kDa
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Figure 1. Western blot analysis of ALAS2/ASB using anti-ALAS2/ASB antibody (A02525-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALAS2/ASB antigen affinity purified polyclonal antibody (Catalog # A02525-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALAS2/ASB at approximately 65 kDa. The expected band size for ALAS2/ASB is at 65 kDa.
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