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Western Blot analysis of K562 cells using CD166 Polyclonal Antibody.
Western Blot analysis of K562 cells using CD166 Polyclonal Antibody.
Western Blot analysis of K562 cells using CD166 Polyclonal Antibody.

ALCAM Antibody

CSB-PA005632
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALCAM
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALCAM Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID214
  • Target name
    ALCAM
  • Target description
    activated leukocyte cell adhesion molecule
  • Target synonyms
    CD166, MEMD, CD166 antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13740
  • Protein Name
    CD166 antigen
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD166/ALCAM using anti-CD166/ALCAM antibody (A01788-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD166/ALCAM antigen affinity purified polyclonal antibody (Catalog # A01788-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD166/ALCAM at approximately 100-110 kDa. The expected band size for CD166/ALCAM is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-CD166/ALCAM Antibody Picoband(r)A01788-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetALCAM
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ReactivityHuman
TargetALCAM
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Product group Antibodies
RayBiotech
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TargetALCAM
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Alcam Polyclonal AntibodyCAC11068
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Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Product group Antibodies
References
Bioss
CD166 Polyclonal AntibodyBS-1251R
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ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetALCAM
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CD166/ALCAM AntibodyCAB2218
ReactivityHuman
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Immunohistochemistry analysis in human parathyroid gland and skeletal muscle tissues using HPA010926 antibody. Corresponding ALCAM RNA-seq data are presented for the same tissues.
Product group Antibodies
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TargetALCAM
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ALCAM / CD166 AntibodyLS-C408399
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CD166 antibody detects CD166 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Green: CD166 stained by CD166 antibody (GTX130098) diluted at 1:500. Red: beta Catenin, a cell membrane marker, stained by beta Catenin antibody [GT3171] (GTX632676) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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