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ALDH1A1 antibody

FNAB00283
Wuhan Fine Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDH1A1
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Overview

  • Supplier
    Wuhan Fine Biotech
  • Product Name
    ALDH1A1 antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Estimated Purity
    ≥95%
  • Gene ID216
  • Target name
    ALDH1A1
  • Target description
    aldehyde dehydrogenase 1 family member A1
  • Target synonyms
    ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1, aldehyde dehydrogenase 1A1, 3-deoxyglucosone dehydrogenase, ALDH class 1, ALHDII, RALDH 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase, liver cytosolic, epididymis luminal protein 12, epididymis luminal protein 9, epididymis secretory sperm binding protein Li 53e, retinal dehydrogenase 1, retinaldehyde dehydrogenase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00352
  • Protein Name
    Aldehyde dehydrogenase 1A1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A1 antigen affinity purified polyclonal antibody (Catalog # A01392) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A1 at approximately 55 kDa. The expected band size for ALDH1A1 is at 55 kDa.
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