![ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110784/GTX110784_43054_20181221_IHC-P_M_w_23060500_249.webp)
ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ALDH1A3 antibody [N2C2], Internal
GTX110784
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDH1A3
Overview
- SupplierGeneTex
- Product NameALDH1A3 antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.1 mg/ml
- ConjugateUnconjugated
- Gene ID220
- Target nameALDH1A3
- Target descriptionaldehyde dehydrogenase 1 family member A3
- Target synonymsALDH1A6, ALDH6, MCOP8, RALDH3, retinaldehyde dehydrogenase 3, acetaldehyde dehydrogenase 6, aldehyde dehydrogenase 6
- HostRabbit
- IsotypeIgG
- Protein IDP47895
- Protein NameRetinaldehyde dehydrogenase 3
- Scientific DescriptionAldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chung SY, Hung YP, Pan YR, et al. Ruxolitinib Combined with Gemcitabine against Cholangiocarcinoma Growth via the JAK2/STAT1/3/ALDH1A3 Pathway. Biomedicines. 2021,9(8). doi: 10.3390/biomedicines9080885Read this paper
- Park JW, Jung KH, Byun Y, et al. ATP-binding Cassette Transporters Substantially Reduce Estimates of ALDH-positive Cancer Cells based on Aldefluor and AldeRed588 Assays. Sci Rep. 2019,9(1):6462. doi: 10.1038/s41598-019-42954-9Read this paper
- Chen MH, Weng JJ, Cheng CT, et al. ALDH1A3, the Major Aldehyde Dehydrogenase Isoform in Human Cholangiocarcinoma Cells, Affects Prognosis and Gemcitabine Resistance in Cholangiocarcinoma Patients. Clin Cancer Res. 2016,22(16):4225-35. doi: 10.1158/1078-0432.CCR-15-1800Read this paper
![ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110784/GTX110784_43054_20181221_IHC-P_R_w_23060500_141.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110784/GTX110784_43082_20191025_WB_TPM_watermark_w_23060500_574.webp)
![ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm. ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110784/GTX110784_43082_20180314_ICC_IF_w_23060500_285.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110784/GTX110784_42992_20171006_WB_w_23060500_115.webp)
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:2000. Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:2000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110784/GTX110784_41605_20170209_WB_w_23060500_832.webp)


![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [GT926] (GTX633822) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633822/GTX633822_42688_20171006_WB_2_w_23061202_702.webp)



