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WB analysis of human stomach tissue lysate using GTX31778 ALDH3A1 antibody. Working concentration : (A) 1 and (B) 2 μg/ml
WB analysis of human stomach tissue lysate using GTX31778 ALDH3A1 antibody. Working concentration : (A) 1 and (B) 2 μg/ml
WB analysis of human stomach tissue lysate using GTX31778 ALDH3A1 antibody. Working concentration : (A) 1 and (B) 2 μg/ml

ALDH3A1 antibody

GTX31778
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDH3A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ALDH3A1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID218
  • Target name
    ALDH3A1
  • Target description
    aldehyde dehydrogenase 3 family member A1
  • Target synonyms
    ALDH3, ALDHIII, aldehyde dehydrogenase, dimeric NADP-preferring, aldehyde dehydrogenase isozyme 3, aldehyde dehydrogenase type III, stomach aldehyde dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30838
  • Protein Name
    Aldehyde dehydrogenase, dimeric NADP-preferring
  • Scientific Description
    Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # A01121-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 55 kDa.
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