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Goat anti-ALDH3A1

EB10119
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetALDH3A1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ALDH3A1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID218
  • Target name
    ALDH3A1
  • Target description
    aldehyde dehydrogenase 3 family member A1
  • Target synonyms
    ALDH3, ALDHIII, aldehyde dehydrogenase, dimeric NADP-preferring, aldehyde dehydrogenase isozyme 3, aldehyde dehydrogenase type III, stomach aldehyde dehydrogenase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000682.3. Purification: Antigen affinity purified. Names and symbols: ALDH3A1; aldehyde dehydrogenase 3 family, memberA1; ALDH3; ALDHIII; MGC10406; aldehyde dehydrogenase 3A1; aldehyde dehydrogenase isozyme 3; aldehyde dehydrogenase type III; aldehyde dehydrogenase, dimeric NADP-preferring; stomach aldehyde dehydrogenase
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # A01121-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 55 kDa.
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